Absolute determination of reduced nicotinamide-adenine dinucleotide by controlled potential coulometry using hexacyanoferrate(III) ion mediator.
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منابع مشابه
Inhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
متن کاملFormation of reduced nicotinamide adenine dinucleotide peroxide.
Incubation of NADH at neutral and slightly alkaline pH leads to the gradual absorption of 1 mol of H+. This uptake of acid requires oxygen and mainly yields anomerized NAD+ (NAD+), with only minimal formation od acid-modified NADH. The overall stoichiometry of the reaction is: NADH + H+ + 1/2O2 leads to H2O + NAD+, with NADH peroxide (HO2-NADH+) serving as the intermediate that anomerizes and b...
متن کاملInhibition of nicotinamidase by nicotinamide adenine dinucleotide.
Feedback inhibition by NAD was shown with the nicotinamidases of a number of microorganisms. The purified enzyme from Fleischmann’s yeast was found to have a molecular weight of 110,000 and consist of presumably identical subunits with a molecular weight of 26,000. A LineweaverBurk plot of the NAD inhibition is concave upward at low concentrations of NAD; at 6 mr+r NAD, the linear plot is that ...
متن کاملSubstituted Nicotinamide Analogues of Nicotinamide Adenine Dinucleotide.
A number of nicotinamide adenine dinucleotide analogues have been prepared in which the purine, pyridine, and ribose moieties have been modified (2-12). These analogues have proven to be valuable in studies dealing with the site of binding of the pyridine coenzyme to dehydrogenases, in elucidating the mechanism of dehydrogenases and the configuration of the pyridme coenzymes, and as indicators ...
متن کاملSubstituted Nicotinamide Analogues of Nicotinamide Adenine Dinucleotide*
A number of nicotinamide adenine dinucleotide analogues have been prepared in which the purine, pyridine, and ribose moieties have been modified (2-12). These analogues have proven to be valuable in studies dealing with the site of binding of the pyridine coenzyme to dehydrogenases, in elucidating the mechanism of dehydrogenases and the configuration of the pyridme coenzymes, and as indicators ...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1993
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.42.5_333